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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: J Chromatogr A. 2009 Oct 2;1217(6):902–924. doi: 10.1016/j.chroma.2009.09.073

Fig. 12.

Fig. 12

Chromatographic separation of three model proteins using monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) capillary column prepared by thermally (A) and photochemically (B) initiated polymerization. Conditions: column 20 cm × 100 μm I.D.; mobile phase A 2% formic acid in 98:2 water:acetonitrile mixture, mobile phase B 2% formic acid in acetonitrile; gradient from 100% A to 50% B in A in 4 min; flow rate 4 μL/min. Peaks: (1) ribonuclease A (2 pmol), (2) cytochrome c (1 pmol), and (3) myoglobin (0.3 pmol). Dashed line represents the overall back pressure in the system. Reproduced from ref. [143] with permission.