Fig. 1.

Site-specific phosphorylation of tau induced by anesthesia. Mouse brains were removed immediately after being sacrificed by cervical dislocation (control), after inhalation of ether vapor for 30 sec, or 5 min after intraperitoneal injection of pentobarbital (50 mg/kg body weight). Homogenates of the forebrains were analyzed by Western blots developed with phosphorylation-dependent tau antibodies for measuring tau phosphorylation at specific sites or with a phosphorylation-independent tau antibody 92e that measures total tau level (A). Each lane represents an individual brain sample. The blots were also quantitated densitometrically (B). The relative immunoreactivities with each phospho-tau antibody, after being normalized with 92e immunoreactivity (total tau), are shown as mean ± SD. ^*p < 0.05 vs. controls.