Table 3.
Serum concentrations of leptin, cytokines, and hyaluronic acid
| Leptin intact | Leptin impaired | Intact v. impaired | ||
|---|---|---|---|---|
| WT (n = 13) | ob/ob (n = 6) | db/db (n = 5) | P | |
| Leptin | 6.06 ± 1.31 | 1.02 ± 0.01 | 169.3 ± 32.9# | ** |
| Pro-inflammatory cytokines | ||||
| IL-1α | 979.4 ± 205.5 | 323.5 ± 61.7 | 1153.6 ± 329.1# | NS |
| IL-1β | 80.9 ± 49.8 | 82.7 ± 48.2 | 76.0 ± 67.9 | NS |
| IL-2 | 151.8 ± 77.0 | 6.0 ± 0.0 | 6.0 ± 0.0 | NS |
| IL-6 | 79.0 ± 37.4 | 6.1 ± 0.0 | 16.7 ± 10.6 | NS |
| IL-17 | 52.6 ± 35.7 | 9.3 ± 0.0 | 95.5 ± 53.1 | NS |
| KC | 243.5 ± 63.7 | 391.8 ± 45.7 | 524.9 ± 57.2 | * |
| TNF-α | 16.5 ± 1.7 | 14.8 ± 0.0 | 70.8 ± 56.0 | NS |
| Anti-inflammatory cytokines | ||||
| IL-4 | 9.4 ± 0.0 | 9.4 ± 0.0 | 21.3 ± 9.4# | NS |
| IL-10 | 91.5 ± 70.6 | 20.9 ± 0.0 | 20.9 ± 0.0 | NS |
| IL-12 | 278.0 ± 50.5 | 214.2 ± 54.6 | 229.9 ± 74.2 | NS |
| Biomarker | ||||
| Hyaluronic Acid (ng/ml) | 431.6 ± 43.8 | 327.5 ± 90.4 | 487.8 ± 283.1 | NS |
Data presented as mean ± standard error of the mean (pg/ml unless otherwise noted). Values below the lowest level of quantification (LLQ) were given a value of 0.5×LLQ. Statistical analyses conducted on log10 values to correct for non-normal distributions.
*
p<0.05,
**
p<0.01.
#
P<0.05 for ob/ob versus db/db comparison (nested ANOVA).