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. Author manuscript; available in PMC: 2011 Mar 28.
Published in final edited form as: Cancer Lett. 2009 Sep 3;289(2):188–194. doi: 10.1016/j.canlet.2009.08.015

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

17-AAG decreases the expression of Hsp-90 client proteins associated with cell growth and proliferation in PDT-treated BA cells. (A) Western immunoblot analysis documenting expression of survivin, phospho-survivin (Thr34), Akt, phospho-Akt (Ser473), Hsp-90, and actin in controls and PDT-treated cells. Cells were incubated with PH (25 µg/ml) in the dark for 16 hr and exposed to red light (210 or 420 J/m2). Cells were then incubated in the dark for an additional 24 hr in the presence or absence of 17-AAG (1 µM). Lysates from control and treated cells were collected and analyzed for protein expression using commercially available antibodies. Actin expression was used to monitor protein loading. Densitometric measurements (averages ± SE) from immunoblots of three separate experiments are shown for Akt (B), phospho-Akt (Ser473) (C), survivin (D), phospho-survivin (Thr34) (E) and Hsp-90 (F).