Table II.
A summary of boronic acid-based sensors
| Compound No. | Sensor Target | Binding Constants (Ka) | Study conditions |
|---|---|---|---|
| 19, 20a–c | Glucose | 23 M−1 to 37 M−1 | λex: 460 nm, λem: 510 nm, in phosphate buffer, pH = 7.4 |
| 21, 20a–c | Glucose | 14 M−1 | λex: 414 nm, λem: 644 nm, in phosphate buffer, pH = 7.4 |
| 22, 20a–c | Glucose | around 100 M−1 | λex: 460 nm, λem: 604 nm, in phosphate buffer, pH = 7.4 |
| 26 | gluconic acid | 107 M−1 | UV studies in 75% methanol/HEPES buffer, pH = 7.4 |
| 27 | sorbitol | 1060 M−1 (5 fold over fructose) | λex = 365 nm, λem = 420 nm, in50% MeOH/aqueous phosphate buffer, pH = 7.4 |
| 28a | glucose | 1472 M−1 (43 fold over fructose) | λex = 370 nm, λem = 430 nm, in aqueous phosphate buffer, pH = 7.4 |
| 30 | ribose | 2400 M−1 (24 fold over fructose, 8 fold over galactose, and 12 fold over glucose) | λex = 565 nm, λem = 597 nm, in90% DMSO/phosphate buffer, pH = 7.4 |
| 34 | D-mannitol | enantioselectivity (KR/KS) of 1:20000 in binding with D-mannitol | λex = 373 nm, λem = 421 nm, in52% CH3OH/aqueous buffer, pH = 8.3. |
| 37 | D-glucose | D-glucose (24800 ± 1200 M 1) to L-glucose (11900 ± 1600 M 1) | λex = 285 nm, λem = 480 nm, in50% CH3OH/water, pH =11.7 |
| 44 | heparin | Heparin (3 × 107 M−1) over dextran (3 × 103 M−1), hyaluronic acid (2 × 103 M−1), chondroitin (4 × 106 M−1), and ovalbumine (1 × 106 M−1). | λem = 510 nm, in HEPES buffer |