Figure 3. Nicotinic effects on IL-10 production by LPS-stimulated U937 cells.
A, B. Differentiated U937 cells were stimulated with LPS in the presence or absence of 1 μM Epi ± 50 μM Mec or 100 nM MLA, or 100 μM AR-R17779 (AR) ± 100 nM MLA and used in the qPCR (A) and ICW (B) assays of the IL-10 gene expression as described in the legend to Fig. 2. Asterisk = p<0.05 compared to LPS given alone; pound sign = p<0.05 compared to the relevant agonist given alone.
C. Concentration-dependent effects of Epi and AR-R17779 on the level of IL-10 protein in differentiated U937 cells stimulated with LPS.
The results are expressed as fold of control determined in control PMA-differentiated U937 cells, and taken as 1.
