Table 1.

Primers used for RT-PCR analysis of nAChRs in differentiated U937 cells^*

Target mRNA	Forward/reverse primers	Product size, bp	Anneal temperature	Sources
α1	CGT CTG GTG GCA AAG CT CCG CTC TCC ATG AAG TT	580	55	[86]
α2	GGA GCT CTG CCA CCC CCT AC AAC ATA CTT CCA GTC CTC	327	64	[87]
α3	CTG GTG AAG GTG GAT GAA GT CTC GCA GCA GTT GTA CTT GA	464	58	[86]
α4	GGA TGA GAA GAA CCA GAT GA CTC GTA CTT CCT GGT GTT GT	444	58	[86]
α5	TCA ACA CAT AAT GCC ATG GC CCT CAC GGA CAT CAT TTT CC	219	64	[87]
α6	GTG GCC TCT GGA CAA GAC AA AAT TAT AAA TAC CCA AAG A	372	58	[86]
α7	CTT CAC CAT CAT CTG CAC CAT C GGT ACG GAT GTG CCA AGG ATA T	308	55	[87]
α9	GTC CAG GGT CTT GTT TGT ATC CGC TCT TGC TAT GAT	403	58	[87]
α10	CTC TCA AGC TGT TCC GTG ACC AAG GCT GCT ACA TCC ACG C	394	64	[88]
β1	TGT ACC TGC GTC TAA AAA GG GCA GGT TGA GAA CCA CGA CA	455	60	[87]
β2	CAG CTC ATC AGT GTG CA GTG CGG TCG TAG GTC CA	347	58	[89]
β3	AGA GGC TCT TTC TGC AGA GCC ACA TCT TCA AAG CAG	354	60	[89]
β4	GTG AAT GAG CGA GAG CAG AT GGG ATG ATG AGG TTG ATG GT	524	58	[86]
δ	CAG ATC TCC TAC TCC TGC AA CCA CTG ATG TCT TCT CAC CA	471	58	[86]
γ	CGC CTG CTC TAT CTC AGT CA GGA GAC ATT GAG CAC AAC CA	546	56	[86]
ε	GTA ACC CTG ACG AAT CTC AT GTC GAT GTC GAT CTT GTT GA	432	55	[86]

^*All products were sequenced by the designers of the PCR primer used in this study.