Figure 1.

Trephining the ONH in the orientation of the acquired B-scan. (A) The SD-OCT IR image including the 15° acquisition box, viewed at 50% opacity, was aligned to an image of the ONH specimen. (B) The coordinates of the acquisition box were burned onto the image. (C) Cuts were made in the specimen, in an attempt to get the closest approximation to the orientation of the acquired B-scans. The cut made in the superior part of the specimen (red arrows) most closely matched the acquired B-scan orientation. The specimen was embedded with the superior cut surface facing downward.