Figure 4.

Method of identifying the B-scan angle of rotation. (A) The topographic orientation of serial hematoxylin and eosin–stained sections is depicted as a series of red lines in the disc photograph. There was a minimum separation of 16 μm between sections. Sections that could not be oriented because of poorly preserved retina account for the variable width of gaps between the lines. (B) The image shown (A, viewed at 50% opacity) was colocalized to the en face SD-OCT image (C-scan). Green lines: 0° and 90° orientations of the actual acquired horizontal raster B-scans. (C) The angle of rotation of the histologic sections assessed relative to that of the acquired B-scans varied within three regions: 73.5° in histologic sections from region 1, 87° in sections from region 2, and 89° in sections from region 3.