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Assessment of Cell proliferation. (A) Hepatocytes expressing PCNA were detected by immunohistochemical assay and then counted from 20 randomly selected fields at ×200 magnification (Insert: typical picture of PCNA positive hepatocytes). (B) Hepatic cyclin D1 protein concentration was measured from whole liver homogenates by using Western blotting. Bars represent means ± SEM, n = 8. * P < 0.05 between groups.
