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. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: J Invest Dermatol. 2009 Feb 26;129(7):1805–1815. doi: 10.1038/jid.2008.435

Figure 5. ATR-induced apoptosis is largely p53-independent in human keratinocytes.

Figure 5

Human keratinocytes were transfected by nucleofection with siRNA against ATR and/or p53, or a non-targeting/scrambled control (scr). Forty-eight hours later, cells were exposed to 75 mJ/cm2 of UVB. (a) Western blots using the indicated antibodies. Cells were harvested 8 h after UVB irradiation. (b) Percentage of sub-2N DNA content measured by flow cytometry. Cells were harvested 24 h after UVB irradiation and stained with propidium iodide. (c) Relative cell death was calculated by comparing percentage of sub-2N DNA content in ATR/UVB-, p53/UVB-, or ATR+p53/UVB-treated cells with that in scr/UVB-treated cells in each experiment. Average of relative cell death is shown (n=3). Error bar, standard error of the mean.