Table 2.
mini-Tn10 transposon mutants identified in the cytochrome c/heme pathways
| Strain Number |
Locus | Name/Annotation | Function |
pagA-lacZ Increase a |
atxA- lacZ Increase b |
TMPD staining c |
|---|---|---|---|---|---|---|
| tB3 | BAS4358* | glutamate-1-semialdehyde-2,1-aminomutase (HemL) |
heme synthesis | + | E | +/− |
| tB17 | BAS4358* | glutamate-1-semialdehyde-2,1-aminomutase (HemL) |
heme synthesis | + | E | +/− |
| tB18 | BAS1385 | ResC | cytochrome c maturation |
++ | E | − |
| tB21 | BAS1384* | ResB | cytochrome c maturation |
++ | E | − |
| tB23 | BAS3568 | conserved hypothetical protein | unknown | ++ | E | +/− |
| tB28 | BAS1384* | ResB | cytochrome c maturation |
++ | E | − |
*
multiple strains with unique transposon insertions into the same gene
a
: + = pagA-lacZ expression higher than parental strain; ++ = pagA-lacZ expression much higher than parental strain
b
: E = induced atxA-lacZ expression in exponential phase
c
: +/− =staining reduced relative to parental strain; − = no staining