FIG. 3.

Baseline mRNA expression of IFN pathway-associated genes among human mesothelioma lines. Total RNA was extracted from the primary mesothelial cell line, PF1M, and 10 human mesothelioma lines and used to synthesize cDNA. Specific primer sets were made to amplify eight IFN pathway-associated genes: (A) IFNAR1, IFNAR2, (B) JAK1, TyK2, (C) STAT1, STAT2, (D) p48, and double-stranded RNA-dependent protein kinase (PKR). Each sample was run in quadruplicate and the sample loading was adjusted in relation to their β-actin or GAPDH expression. The expression of each gene is normalized to the expression in the PF1M line and the fold change ± standard deviation is shown.