Table 2.
Upregulation of Interferon-β mRNA and Protein in Human Mesothelioma Cells After Infection with Vesicular Stomatitis Virus Expressing Green Fluorescent Protein at an MOI of 10 for 6 hr, Measured Using Real-Time Reverse Transcriptase–Polymerase Chain Reaction, or 24 hr, Using Interferon-β Bioassay
| |
mRNA levels (fold change) |
Interferon-β protein levels (U/ml/million cells) |
||
|---|---|---|---|---|
| Cell lines | Control | VSV.GFP infecteda | Control | VSV.GFP infected |
| LP9 | u/d | 7325% ± 6%b | u/d | 0.225 |
| REN | u/d | 11,428% ± 12.5% | u/d | 0.007 ± 0.003 |
| LRK | u/d | u/dc | u/d | u/d |
Abbreviations: LP9, normal mesothelial cells; u/d, undetectable; VSV.GFP, vesicular stomatitis virus expressing green fluorescent protein.
Levels of mRNA are expressed in arbitrary units.
Expression levels are presented as means of triplicates with ± the coefficient of variation (standard error of the mean/mean).
p-Value is < 0.05 compared with LP9.