Table 3B.
Incubation with Vesicular Stomatitis Virus Expressing Green Fluorescent Protein at an MOI of 10 for 6 hr
| |
MxA mRNAa |
2′5′-OAS mRNA |
PKR mRNA |
|||
|---|---|---|---|---|---|---|
| Cell lines | Control | VSV.GFP infected | Control | VSV.GFP infected | Control | VSV.GFP infected |
| LP9 | u/d | 82% ± 2.2%b | u/d | 105% ± 1.7% | 615% ± 9.1% | 958% ± 1.7% |
| REN | 13% ± 12.2% | 30% ± 5.9%c | u/d | 23% ± 6.3%c | 45% ± 9.7% | u/d |
| LRK | u/d | u/d | u/d | u/d | u/d | u/d |
Abbreviations: MxA, myxovirus resistance A; 2′5′-OAS, 2′,5′-oligo-adenylate-synthetase; PKR, double-stranded RNA-dependent protein kinase; VSV.GFP, vesicular stomatitis virus expressing green fluorescent protein.
a
Levels of mRNA are expressed in arbitrary units based on a standard curve.
b
Expression levels are presented as means of triplicates with ± the coefficient of variation (standard error of the mean/mean).
c
p-Value is < 0.05 compared with LP9.