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. 2009 Dec 17;21(1):40–50. doi: 10.1089/hum.2009.027

FIG. 2.

FIG. 2.

Production of LV via HL vector system. (A) Production of LV in a variety of cell types after HL infection. Various cell lines indicated in the figure were infected with HL at multiplicity of infections (MOIs) of 1, 10, or 100. After 48 hr, viral supernatant was collected and titrated on 293 cells for βgal expression. (B) Production of LV following HL vector infection. Hep3B cells were infected with the HL vector at MOIs of 0.1, 1, 10, or 100. After 48 hr, viral supernatant was collected and titrated on 293 cells for βgal expression in the presence or absence of zidovudine (AZT, 5 μM). Data shown are average titers and standard deviations from the experiment performed in triplicate. Effect of AZT on titers was determined by Student's t-test (**p < 0.05, *p < 0.01). (C) Time course of lentiviral production from Hep3B cells infected with the HL vector. Hep3B cells were infected with HL at an MOI of 10 and monitored for up to 6 days. At different time points indicated in the figure, the medium was replaced, and the viral supernatant was titrated for βgal expression on 293 cells.