FIG. 3.

Spread of LV-transduced cells and persistent gene expression following HL vector infection. (A) Transduction efficiencies of HL hybrid vector-infected cells. Hep3B cells (2 × 10⁵) infected with the HL vector at an MOI of 10 were incubated overnight, and the cells were split the following day and cultivated in the presence (blue circle) or absence (red triangle) of AZT on a 10-cm dish. The control samples infected with an LV GFP (brown square) or an Ad GFP (green diamond) are also shown. The cells were passaged at a ratio of 1:20 every week, and expressions of GFP and βgal were examined. Data are representative of three independent experiments, all yielding similar results. (B) Persistent gene expression achieved via HL hybrid vector system in transformed human hepatocytes in vitro. Hep3B cells were infected with HL vector at an MOI of 10. The cells were passaged at a ratio of 1:20 every week. Expressions of GFP and βgal were analyzed by immunofluorescence staining using anti-GFP and anti-βgal antibody at the indicated time points after HL infection.