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. 2009 Dec 17;21(1):40–50. doi: 10.1089/hum.2009.027

FIG. 4.

FIG. 4.

In vivo transduction in humanized liver of the chimeric mice via HL hybrid vector system. The mice were injected with HL vector or phosphate-buffered saline (PBS) buffer. At indicated time points following HL infection, liver tissue was analyzed for GFP and βgal expression as follows: (A) Human CK8/18 immunostaining to determine replacement index of the mouse liver with human hepatocytes (human hepatocytes appear brown). Original magnification: 10 ×. (B) Immunofluorescence stain for GFP. Original magnification: 200 ×. (C) Immunohistochemical stain for GFP. Original magnification: 200 ×. (D) Immunofluorescence staining for βgal. Original magnification: × 200. (E) X-gal staining. Original magnification: × 100. Representative sections of each stain are shown.