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. 2009 Dec 17;21(1):40–50. doi: 10.1089/hum.2009.027

FIG. 5.

FIG. 5.

Detection of integrated second-stage LV in liver tissue from chimeric mice after HL vector administration. (A) Design of nested polymerase chain reaction (PCR) analysis to amplify sequences spanning adjacent Alu repeats in the human genome (Alu-s and 5NC2-as) and the integrated lentiviral LTR (LTR9-s and U5PBS-as) (Nguyen et al., 2002; Serafini et al., 2004). (B) Result of nested PCR analysis: PCR1 and PCR2 correspond to the first and second rounds of nested PCR, respectively. M, 1-kb molecular mass size ladder (Invitrogen); lane 1, PBS-treated; lane 2, HL-infected, 4 days postinfection; lane 3, HL-infected, 4 weeks postinfection; lane 4, no DNA template. The 121-bp final amplification product is indicated. A 500-bp region of the human β-actin gene was amplified from the same samples as an internal control.