FIG. 1.

Intraperitoneal injection of AAV-CMV-LacZ leads to neonatal spinal cord transduction. (A) LacZ-positive cells in white matter. About 5 × 10¹² vector genomes of dsAAV8-CMV-LacZ were delivered on 3-day-old neonatal mice, and the mice were sacrificed at 2 months of age. Notably, most of the lacZ-positive spots were in white matter (black arrowheads and insets), and very few LacZ-positive cells were in gray matter (black arrows). Insets show enlarged area. The consecutive sections of H&E staining were used to display the structure of spinal cord. It was also apparent that there were more LacZ-positive cells in lower spine, followed by medium spine, and then the upper one. (B) LacZ-positive cells in gray matter. Button-like structures (black arrow) in gray matter indicate small neuron and axonal communication. AAV, adeno-associated virus; CMV, cytomegalovirus; H&E, hematoxylin and eosin. Color images available online at www.liebertonline.com/hum.