FIG. 3.

Immunofluorescent staining of GFP in spinal cord, peripheral nerves, and DRG demonstrates that those cells could be efficiently transduced by AAV8-GFP vector in neonates. Similar to AAV8-CMV-LacZ vector, the 2 × 10¹² vector genomes/ml dsAAV8-CMV-GFP vector was also delivered into neonatal mice by intraperitoneal injection (2 × 10¹¹ vector genomes/neonatal mouse). Two months after vector delivery, the mice were sacrificed. (A) Transduction of white matter of spinal cord and DRG by dsAAV-CMV-GFP vector. The same as the results obtained from LacZ staining, most GFP-positive signals (white arrow) were located in white matter of spinal cord and DRG. The neurons located in DRG transduced by AAV vector are about 70–80%. The consecutive sections of H&E staining were used to display the structure. (B) Transduction of peripheral nerves by dsAAV8-CMV-GFP vector. Insets show enlarged area. GFP, green florescent protein.