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. 2009 Dec 17;21(1):87–97. doi: 10.1089/hum.2009.131

FIG. 4.

FIG. 4.

FIG. 4.

Intramuscular injection of AAV-CMV-LacZ leads to spinal cord transduction. (A) LacZ-positive cells in spinal cord of vector-delivered mice (10 × ). The vector was delivered on 3-week-old adult mice and the mice were sacrificed 1, 2, or 3 months after injection. Obviously, most of the lacZ-positive spots were in white matter (WM; black arrowheads and insets), and very few LacZ-positive cells were in gray matter (GM; black arrows). Insets show enlarged areas of WM (40 × ). LFB staining was used on consecutive sections to display the structure of spinal cord. (B) LacZ staining of brain stem in AAV8-CMV-LacZ-delivered adult mice. The mice were sacrificed 3 months after intramuscular injection. Some LacZ-positive cells were found in the WM of the dorsal part of the left upper brain stem (inset area), most likely in the gracile fasciculus and cuneate fasciculus. Inset shows the enlarged area (40 × ). The consecutive section of LFB staining was used to display the structure of the brain stem. LFB, luxol fast blue. Color images available online at www.liebertonline.com/hum.