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. 2010 Feb 4;7:28. doi: 10.1186/1743-422X-7-28

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Copyright ©2010 Schieffelin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of the ConA ELISA. DENV E protein from serotypes 1-4 derived from DENV infected LLC-MK-2 cells was immobilized by ConA. Reactivity with patient serum and dengue negative human serum diluted in PBS/Tween as well as MMAbs 4G2 and 3H5 was measured by detection with HRP anti-human and anti-mouse IgG, respectively. MMAb 4G2 were tested at 5 ug/ml. Mouse MAb 3H5 was tested as undiluted culture fluid. Negative controls of uninfected LLC-MK-2 culture fluid and Vaccinia ADA were also tested.