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Cross-competition between HMAbs. A cross-competition assay was performed to determine whether the three HMAbs and MMAb 4G2 recognized overlapping or non-overlapping sties on DENV-1 E protein. Purified MMAb 4G2 and HMAbs were incubated with biotinylated HMAbs, washed, and the presence of biotinylated antibodies was detected using streptavidin.
