Figure 2.

Electroporation mediate plasmid transfection of quadriceps. a) In vivo muscle transfection by EP was assessed by luciferase activity in resected leg 72 h post transfection (imaged for 1 min using an intensified CCD camera (IVIS Imaging System, Xenogen). b) RT-PCR analysis of mRNA expression of hPSA in muscle. hPSA was only detected in muscles electroporated with phPSA (lane 1 100 bp marker, lane 2 phPSA transfected muscle (sample a), lane 3 phPSA transfected muscle (sample b), lane 4 empty vector transfected muscle, lane 5 untreated muscle).