Figure 1.

Procedures for immunofluorescent staining and Western blotting on mouse somatic nuclei transplanted to Xenopus laevis oocyte germinal vesicles (GVs). Mouse somatic cells were permeabilized and injected to GVs. Injected oocytes were cultured for the desired time. Whole oocytes were collected for isolating RNA or for chromatin immunoprecipitation assay. For immunofluorescent staining or Western blotting, injected oocytes were manually dissected to isolate GVs. The isolated GVs were either fixed to be stained or dispersed by vortexing to collect only injected somatic nuclei.