Table 2.

Comparison of average lifespan

Compared to	Days change	Percent change
Does cold temperature change average lifespan?
25°C, WT, ad liba	16.70	104.71
25°C, WT, DRa	21.19	86.19
25°C, clk-1, ad liba	17.49	102.29
25°C, clk-1, DRa	34.66	108.83
25°C, daf-2, ad lib	13.27	41.39
25°C, daf-2, DRa	12.13	28.71
25°C, clk-1;daf-2, ad liba	17.28	49.33
25°C, clk-1;daf-2, DRa	12.75	25.15
Does daf-2 change average lifespan?
25°C, WT, ad liba	16.12	101.11
16°C, WT, ad lib	12.70	38.90
25°C, WT, DRa	17.66	71.86
16°C, WT, DR	8.61	18.81
25°C, clk-1, ad liba	17.94	104.88
16°C, clk-1, ad liba	17.73	51.24
25°C, clk-1, DRa	18.86	59.20
16°C, clk-1, DR	−3.06	−4.60
Does clk-1 change average lifespan?
25°C, WT, ad lib	1.16	7.25
16°C, WT, ad lib	1.95	5.98
25°C, WT, DRa	7.27	29.58
16°C, WT, DR	20.75	45.34
25°C, daf-2, ad lib	2.97	9.26
16°C, daf-2, ad lib	6.98	15.40
25°C, daf-2, DRa	8.46	20.04
16°C, daf-2, DR	9.09	16.71
Does DR change average lifespan?
25°C, WT, ad liba	8.64	54.16
16°C, WT, ad liba	13.13	40.21
25°C, daf-2, ad lib	10.18	31.74
16°C, daf-2, ad lib	9.04	19.93
25°C, clk-1, ad liba	14.75	86.26
16°C, clk-1, ad liba	31.92	92.28
25°C, clk-1;daf-2, ad liba	15.67	44.73
16°C, clk-1;daf-2, ad lib	11.14	21.29

WT wild type, DR dietary restriction

^aSignificant difference