. Author manuscript; available in PMC: 2011 Jan 15.

Published in final edited form as: Anal Chem. 2010 Jan 15;82(2):548–558. doi: 10.1021/ac901790q

Table 1.

MS methods for analysis of AccQ•Tag derivatized physiological amino acid standards by UPLC-MS/MS using five multiple reaction monitoring functions. Experimental conditions: 2.1 × 100 mm AccQ•Tag colum; 0.25 μmol/mL AccQ•Tag derivatized physiological amino acids, 1 μL sample injection volume; positive ionization mode.

Function #	MRM Transitions	Time window (min)	Dwell time (s)
Function #	MRM Transitions	Time window (min)	Method #1	Method # 2
1	11	1-3	0.01	0.02
2	18	2.5-4.5	0.01	0.02
3	13	4.1-6.5	0.01	0.02
4	17	6.1-7.5	0.01	0.02
5	11	7.2-10	0.01	0.02