Table 3.

Amino acids concentration in P. falciparum rings and trophozoites calculated from biological replicates using the absolute peak areas. Experimental conditions were the same as in Table 1.

Amino Acid	P. falciparum rings			P. falciparum trophozoites			p- value
	pmol/μL	STError	Normalized amount (picomol AA per nanomole of hemozoin)	pmol/μL	STError	Normalized amount (picomol AA per nanomole of hemozoin)
Serine	3.9126	0.6751	7.8252	52.0277	22.8780	34.3499	0.032
Glutamic acid	21.9737	2.5666	43.9474	108.8350	37.7964	71.8554	0.026
Aspartic acid	6.1297	2.5678	12.2594	129.5683	38.5124	85.5440	0.003
Glycine	6.6905	1.3546	13.3810	61.9637	26.0617	40.9099	0.031
Ethanolamine	< LOQ	-	-	1.6462	1.3428	1.0869	-
Threonine	2.9726	0.4360	5.9452	35.8404	15.5397	23.6627	0.031
Proline	0.3824	0.3412	0.7658	16.9832	7.5931	11.2127	0.032
γ-Amino-n- butyric acid	2.0109	0.2745	4.0228	20.2868	6.5311	13.3938	0.007
Alanine	7.6203	0.7733	15.2416	48.2945	20.5785	31.8851	0.034
Tyrosine	< LOQ	-	-	21.2450	10.5872	14.0264	-
Methionie	0.9178	0.1356	1.8366	15.5779	6.1767	10.2849	0.016
Valine	4.5380	0.4064	9.0760	40.0906	17.6434	26.4687	0.032
Tryptophan	0.3127	0.0741	0.6254	3.3853	1.5284	2.2351	0.038
Phenylalanine	1.3687	0.1482	2.7374	15.1528	6.3066	10.0042	0.027
Isoleucine	0.8788	0.1922	1.7586	28.7275	13.6388	18.9666	0.030
Leucine	4.0228	0.3828	8.0466	46.4224	20.8196	30.6491	0.031

Note: LOQ refers to the limits of quantitation.