Table 1.

Oligonucleotide primer sequences and expected PCR product size.

Multiplex Primer Set	VZV ORF	Forward PCR primer (5' - 3')^a	Reverse PCR primer (5' - 3')^b	PCR Product Size (bp)
A	33	AGGTGACACTATAGAATAtgctgaagctgacgaagaga	GTACGACTCACTATAGGGAcgcacaatctctggggtaat	142
A	18	AGGTGACACTATAGAATAtttacatttttatcggcggc	GTACGACTCACTATAGGGAcctcgatgcactcttgttca	152
A	9	AGGTGACACTATAGAATAgcccgtttgagacatgaact	GTACGACTCACTATAGGGAcgcttcggtgaatcctctaa	162
A	51	AGGTGACACTATAGAATAacagctgcttgaccgatttt	GTACGACTCACTATAGGGActattggacatgcaagggct	172
A	37	AGGTGACACTATAGAATAtgggttcccttcgtacagac	GTACGACTCACTATAGGGAatcatggttttaaatgccgc	182
A	63	AGGTGACACTATAGAATAtcagaggagagcaccgattc	GTACGACTCACTATAGGGActttcgcggctgtatattcc	191
A	58	AGGTGACACTATAGAATAgcgttgatgcaaatgaaaaa	GTACGACTCACTATAGGGAaggcgttcataaccaagctg	202
A	32	AGGTGACACTATAGAATAcatcatccgtccaaacagtg	GTACGACTCACTATAGGGAaaggtttcgttgcacatgct	212
A	62	AGGTGACACTATAGAATAgacagcccagtggagaaaaa	GTACGACTCACTATAGGGAgaatcatccgaatttgacgg	222
A	41	AGGTGACACTATAGAATAcccagaccttaatgcgagaa	GTACGACTCACTATAGGGAaaaccaatgtgcgaatagcc	232
A	42	AGGTGACACTATAGAATAaccgttgcgaattgtgctat	GTACGACTCACTATAGGGAgtaacgcaacgccctatctc	237
A	44	AGGTGACACTATAGAATAgagtgtggtggtcagacgaa	GTACGACTCACTATAGGGAttccgcatgatttgtgacat	246
A	29	AGGTGACACTATAGAATAgtttataggcatggagggca	GTACGACTCACTATAGGGAggattcgcagctaaatgagg	256
A	54	AGGTGACACTATAGAATAgatcggtggacacatcacaa	GTACGACTCACTATAGGGAccgtaaccgtttcagctctc	276
A	38	AGGTGACACTATAGAATAtgttcttaccactcccccag	GTACGACTCACTATAGGGAcaatgggaattgatgatccc	286
A	3	AGGTGACACTATAGAATAgttggcgtcgtttacacaag	GTACGACTCACTATAGGGAcggtaacagtcgttgggaat	296
A	28	AGGTGACACTATAGAATAaagctttcgcgttgaggtta	GTACGACTCACTATAGGGAtccgacatgcagtcaatttc	343
B	1	AGGTGACACTATAGAATAgttcgggaatctgatagcga	GTACGACTCACTATAGGGAgtggtcttgtttgaacgacg	137
B	15	AGGTGACACTATAGAATAgtccatgatgaaaacctggg	GTACGACTCACTATAGGGAgctgaggttattgcacacga	147
B	49	AGGTGACACTATAGAATAatctgtggattgtgcaaacg	GTACGACTCACTATAGGGAatcggcgtcctctgttacat	157
B	61	AGGTGACACTATAGAATAgaaagggatacccgattggt	GTACGACTCACTATAGGGAatcgtttaacgtctctgccg	167
B	24	AGGTGACACTATAGAATAgcccgttgtgtttttcagtt	GTACGACTCACTATAGGGAacagacacccccgtattttg	177
B	68	AGGTGACACTATAGAATAccgagacttggagctttttg	GTACGACTCACTATAGGGAcggccaactgatcctcttta	187
B	53	AGGTGACACTATAGAATAttactgaaccctcagcctgg	GTACGACTCACTATAGGGAccgaatctcgtaaaggtcca	197
B	4	AGGTGACACTATAGAATAtccggggataaaacaaaaca	GTACGACTCACTATAGGGAacagttcatccgcagactcc	207
B	64	AGGTGACACTATAGAATAcgctatgcgccatatctacc	GTACGACTCACTATAGGGAtaacgataacaccgctctgc	216
B	57	AGGTGACACTATAGAATAcgagaacgtaatgtgtttggaa	GTACGACTCACTATAGGGAacgttgatgagccttgcag	241
B	48	AGGTGACACTATAGAATAtcggaacgtatgaatgtgga	GTACGACTCACTATAGGGAttaacgtggtgtacgtccga	256
B	50	AGGTGACACTATAGAATAccacggttaattcccacaac	GTACGACTCACTATAGGGAacgtagccgtagccttgtga	270
B	20	AGGTGACACTATAGAATAcgtggtattggcttcctgtt	GTACGACTCACTATAGGGAtgcaggataattgtgtggga	281
B	67	AGGTGACACTATAGAATAatacggtggcgttttgtttc	GTACGACTCACTATAGGGAaccaagaatgaaaccatcgg	291
B	10	AGGTGACACTATAGAATAgttcacgtgtgcgtttcatc	GTACGACTCACTATAGGGAtgaggatgtttgaccgcata	301
B	2	AGGTGACACTATAGAATAtgggtatgaatgtggtacgg	GTACGACTCACTATAGGGAtgttagggttgggagagtgg	305
B	13	AGGTGACACTATAGAATAtgggagacttgtcatgttgg	GTACGACTCACTATAGGGActttagcggcgagttctttg	313
B	7	AGGTGACACTATAGAATAacttacgctgcatgttctcg	GTACGACTCACTATAGGGAtcaaacgatgcctgaggttt	333
C	25	AGGTGACACTATAGAATAgtacgaatcggaaaatgcgt	GTACGACTCACTATAGGGAcgggtagaatcagaagaaccc	142
C	18	AGGTGACACTATAGAATAtttacatttttatcggcggc	GTACGACTCACTATAGGGAcctcgatgcactcttgttca	152
C	55	AGGTGACACTATAGAATAacaacccggactttctgttg	GTACGACTCACTATAGGGAtcgcgatttgaatgtgtttc	157
C	56	AGGTGACACTATAGAATAcggttctgacccaacatctc	GTACGACTCACTATAGGGAcgtcaggtctgtttacccgt	162
C	43	AGGTGACACTATAGAATAcccgataacgccttaacaaa	GTACGACTCACTATAGGGAatcaaagaataaaccggggc	172
C	14	AGGTGACACTATAGAATAcatgccttgttacatgcgtc	GTACGACTCACTATAGGGAttcaatatgaagatcggggc	182
C	45	AGGTGACACTATAGAATAatgtttggcgcgtttctatc	GTACGACTCACTATAGGGAgctgcttcttccggtgaata	192
C	6	AGGTGACACTATAGAATAgcgtgatcgcgaatatacct	GTACGACTCACTATAGGGAtgatccactcgcagagacac	202
C	32	AGGTGACACTATAGAATAcatcatccgtccaaacagtg	GTACGACTCACTATAGGGAaaggtttcgttgcacatgct	212
C	35	AGGTGACACTATAGAATAttatgctagcgtcgcatttg	GTACGACTCACTATAGGGAtaaatccaccccacaagctg	222
C	41	AGGTGACACTATAGAATAcccagaccttaatgcgagaa	GTACGACTCACTATAGGGAaaaccaatgtgcgaatagcc	232
C	60	AGGTGACACTATAGAATAtcatcggtatatgaggcgtg	GTACGACTCACTATAGGGAccccgcttttatgttcttca	242
C	31	AGGTGACACTATAGAATAccgtgggattattggttttg	GTACGACTCACTATAGGGAcgacggttcagtgttttgtg	252
C	59	AGGTGACACTATAGAATAtgctgaataccacgcttacg	GTACGACTCACTATAGGGAgaaatggaacacgggacaat	262
C	26	AGGTGACACTATAGAATAtaccgatggagggttaccag	GTACGACTCACTATAGGGAcgcaatctttccaattagcc	273
C	5	AGGTGACACTATAGAATAactcaacgtttttggatggc	GTACGACTCACTATAGGGAgaatggcccaaacatacgac	282
C	65	AGGTGACACTATAGAATAaaaacaccatggagggtgag	GTACGACTCACTATAGGGAgctcccgtcatagcaaataca	293
C	13	AGGTGACACTATAGAATAtgggagacttgtcatgttgg	GTACGACTCACTATAGGGActttagcggcgagttctttg	313
C	7	AGGTGACACTATAGAATAacttacgctgcatgttctcg	GTACGACTCACTATAGGGAtcaaacgatgcctgaggttt	333
D	40	AGGTGACACTATAGAATAaaccctctgtttgcatgtcc	GTACGACTCACTATAGGGAgaaaaggagggattggtggt	137
D	52	AGGTGACACTATAGAATAaacaacggattccaccgtta	GTACGACTCACTATAGGGAaatacgcaaagattggacgg	147
D	19	AGGTGACACTATAGAATAgagccgatatccttcgaaca	GTACGACTCACTATAGGGAgcagcactgcgtatgattgt	167
D	21	AGGTGACACTATAGAATAatgcagatggatatcgcaca	GTACGACTCACTATAGGGAgccaacagtccgatgaagtt	177
D	34	AGGTGACACTATAGAATAcaatatgaacggggacttgg	GTACGACTCACTATAGGGAggaggcaaactgaggaatga	187
D	12	AGGTGACACTATAGAATAtaccggggaatatgtcagga	GTACGACTCACTATAGGGAataacatccacccgtagcca	197
D	66	AGGTGACACTATAGAATAtttggcaaaacggtcttctc	GTACGACTCACTATAGGGAcgggaagagtttggaaaaca	207
D	27	AGGTGACACTATAGAATAatccattttggcgctatcag	GTACGACTCACTATAGGGAtacatcataaaccctccggc	217
D	30	AGGTGACACTATAGAATActgttttccattgtggggag	GTACGACTCACTATAGGGAttctggtcgatccctgtttc	227
D	39	AGGTGACACTATAGAATAcgatcagacctgctaccctt	GTACGACTCACTATAGGGAccccacgacacaggaagtat	237
D	17	AGGTGACACTATAGAATAgttcgctgtcagaccgattt	GTACGACTCACTATAGGGAtgcatatttggaaacggaca	247
D	8	AGGTGACACTATAGAATAgaagatcatcgaaaagccga	GTACGACTCACTATAGGGAttaccgcggatagcataagg	257
D	46	AGGTGACACTATAGAATAaaagaacggaccctcgatct	GTACGACTCACTATAGGGAgcgaataaaatcgggtgcta	277
D	22	AGGTGACACTATAGAATAttgggggttacgtttactgc	GTACGACTCACTATAGGGAggagtcgatgtacggcaaat	287
D	23	AGGTGACACTATAGAATAcgtacgtttaacccgcgtat	GTACGACTCACTATAGGGAagtgcttgactgtgctccct	298
D	36	AGGTGACACTATAGAATAggcgtatggaattggaaaaa	GTACGACTCACTATAGGGAgtccatcaatgccgagattt	308
E	50	AGGTGACACTATAGAATAtcctggctacgttaattgcc	GTACGACTCACTATAGGGAccatctagggtggcgttaaa	137
E	65	AGGTGACACTATAGAATAgcgagcgcaaccaaataata	GTACGACTCACTATAGGGAgcaaaaattcatctgccgtt	144
E	54	AGGTGACACTATAGAATAatgggcgagaacgatacaac	GTACGACTCACTATAGGGAggaacgtacggtttcggtaa	151
E	11	AGGTGACACTATAGAATAgcagcaagtcctctttacgc	GTACGACTCACTATAGGGAtcctgtcttgctctttgggt	158
E	7	AGGTGACACTATAGAATAactcaaaacggcttgctgat	GTACGACTCACTATAGGGAcacttcactttcagcggtca	165
E	47	AGGTGACACTATAGAATAggacaactgcccatttcagt	GTACGACTCACTATAGGGAggggatgtcagatgggagta	172
E	61	AGGTGACACTATAGAATAcaaacctggacctggaaaga	GTACGACTCACTATAGGGAaaagcctgactttttggggt	179
E	29	AGGTGACACTATAGAATAgcccaaattctgcgttgtat	GTACGACTCACTATAGGGAgtggccatctcgatctgttt	186
E	5	AGGTGACACTATAGAATAtttttggatggcctatctgg	GTACGACTCACTATAGGGActgcacgcaccaaaaataag	193
E	2	AGGTGACACTATAGAATActggaggtgcaaggacattt	GTACGACTCACTATAGGGAttaccccgccataaacagag	200
E	26	AGGTGACACTATAGAATAggagacgttttggcgttaga	GTACGACTCACTATAGGGAaattcaaaggcagcgctaaa	207
E	64	AGGTGACACTATAGAATAcggctgatattgatggcata	GTACGACTCACTATAGGGAcgtaggttcttgggactcca	215
E	16	AGGTGACACTATAGAATAttaacacgggatgagacgtg	GTACGACTCACTATAGGGAttcacagattccatgttcgc	222
E	3	AGGTGACACTATAGAATAagtcaacccatccgagtgaa	GTACGACTCACTATAGGGAagcgtgtaaatcatcagccc	229
E	13	AGGTGACACTATAGAATAggtcaaccgattccaaagaa	GTACGACTCACTATAGGGAgctgatcgattccttgctgt	238
E	42	AGGTGACACTATAGAATAttttatatcggccgtcaacc	GTACGACTCACTATAGGGAcgccctatctcactggaaga	245
E	62	AGGTGACACTATAGAATAacccagaacggaagatgttg	GTACGACTCACTATAGGGAgttcatcggtggttttggag	254
E	63	AGGTGACACTATAGAATAacgcgagattcacgaagatt	GTACGACTCACTATAGGGAacattccagtgcgctcctat	265

Multiplex Set	Cell Gene (accession number)	Forward PCR primer (5' - 3')^c	Reverse PCR primer (5' - 3')^d	PCR Product Size (bp)

A, B, C, D, E	GAPdH (NM_002046 )	AGGTGACACTATAGAATAatcactgccacccagaagac	GTACGACTCACTATAGGGAacctggtgctcagtgtagcc	339
A, B, C, D, E	Beta-actin (NM_001101	AGGTGACACTATAGAATAgctatccctgtacgcctctg	GTACGACTCACTATAGGGAgtcaggcagctcgtagctct	353
A, B, C, D, E	Cyclophilin (BC000689)	AGGTGACACTATAGAATAcctaaagcatacgggtcctg	GTACGACTCACTATAGGGAaggatactgcgagcaaatgg	357

forward universal primer sequence (upper case) and VZV gene-specific primer sequence (lower case) in multiplex primer sets A–E

reverse universal primer sequence (upper case) and VZV gene-specific primer sequence (lower case) in multiplex primer sets A–E

forward universal primer sequence (upper case) and cell gene-specific primer sequence (lower case) in multiplex primer sets A–E

reverse universal primer sequence (upper case) and cell gene-specific primer sequence (lower case) in multiplex primer sets A–E