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The effect of GSH on the consumption of oxygen.
| Conditions | NO added (nmol) |
O2 consumed (nmol) |
NO/O2 |
|---|---|---|---|
| NO | 267 | 62.3 ± 3.0 (4) | 4.32 ± 0.21 |
| NO + GSH | 267 | 81.3 ± 2.4 (4) | 3.25 ± 0.05 |
| NO + CPTIO | 267 | 2.3 ± 3.3 (4) | 0 |
| NO + GSH + CPTIO | 267 | 48.6 ± 4.8 (4) | 5.54 ± 0.55 |
NO gas was added into a buffer solution (50mM phosphate/1mM DTPA, pH 7.4) with and without GSH (1mM) and CPTIO (1mM). Oxygen consumption upon NO addition was monitored by an oxygen electrode at 37°C. Data represent mean ± SD (n=4).
