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Hepatic Nrf2 protein level
Nrf2 protein levels were measured by Western blot analysis. Liver nuclei proteins were subjected to SDS-polyacrylamide gels and electrically transferred to polyvinylidene fluoride membrane for immunostaining with anti-human Nrf2 antibody. Numbers in parenthesis show the expression ratio to wild-type. ##: significantly different from wild-type mice (P<0.01)
