Table 2.

The conditions of the screens along with the relevant OBOC library.

Screen	[bCAII]	Time; Temp	OBOC library (all methionine linked)	%Hits; # Hits sequenced
1gen_1°L	10 nM bCAII	15 hr; 25°C	5-mer library comprehensive in all d- stereoisomers of the natural amino acids except m,c.	121 hits; ∼ 0.02%
2gen_1°L	10 nM bCAII	15 hr; 25°C	6-mer library comprehensive in r, y, k, q, h, f, w, s, l, i, v.	60 hits; ∼ 0.02%
1gen_2°L	1 nM bCAII + 20 μM lhrywf with 4- azidobutanoic acid coupled at N-terminus	18 hr; 25°C	6-mer library comprehensive in all d- stereoisomers of the natural amino acids except m,c; 5-pentynoic acid coupled at N-terminus	146 hits; ∼ 0.024%
2gen_2oL	1 nM bCAII + 20 μM lhrywf with 4-azidobutanoic acid coupled at N-terminus	18 hr; 25°C	P-×6×5×4×3×2×1-m(100%)-TentaGel (P = 5-pentynoic acid; ×6 = v,i,l,f,k,t,w,r; ×5 = f,l,r,k,v,i,n,a; ×4 = f,y,l,k,r,v,i,w; ×3 = f,w,l,k,v,r,y,a; ×2 = k,r,l,f,v,w,s,p; ×1 = k,r,w,f,s,y,l,i.	58 hits; ∼ 0.01%