Table 4.

Hybridization and PCR results in infected blood

PCR Intensity of TCZ amplicons	Total PCR positive	T. cruzi-PCR positive by lineage-specific primers					Hybridization of Trypanosoma cruzi PCR mini-exon and 24Sα rRNA probes
		24Sα rRNA	Intensity of mini-exon amplicons				n = 24		n = 20
		No. (%) positive	Strong	Average	Weak	Total, no. (%)	Mini-exon positive	Mini-exon negative	24Sα rRNA positive	24Sα rRNA negative
Weak	1	1 (100)	0	0	0	0	0	0	0	0
Average	28	21 (75)	1	10	3	14 (50)	1	12	6	1
Strong	25	12 (48)	4	12	2	18 (72)	3	6	12	1
TCZ/121–122δ	4	4 (100)	0	2	0	2 (50)	0	2	0	0
Total	58	38 (65)	5	24	5	34 (59)	4	20	18	2

Hybridization by primers TCZ1–TCZ2 was not detectable and hybridization by primers 121–122 was detectable.