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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Immunol. 2010 Jan 18;184(4):1710–1720. doi: 10.4049/jimmunol.0902796

Figure 2. Identification of IL-23R expressing γδ T cells.

Figure 2

(A) IL-23R (GFP) expression was analyzed on γδ T cells from LNs, spleen, Lamina Propria (LP), liver and Peritoneal Exudate Cells (PEC) in IL-23R-GFP.KI naïve mice. (B, C) LN cells from IL-23R-GFP.KI naïve mice were collected and analyzed for δ-TCR and Vγ1 Vγ4 and Vγ7 (B) or CD27 or CD122 (C) expression on γδ T cells. (D) LN cells collected from IL-23R-GFP.KI or IL-23R−/− were stimulated with PMA/Ionomycin and intracellular cytokine staining for IFN-γ and IL-17 was performed. The quadrants represent intracellular cytokine staining and IL-23R (GFP) expression on γδ T cells. (E) Single cell suspensions were prepared from LNs from WT, β2M−/− or IL-6−/− mice. Quadrants represent intracellular cytokine staining for IL-17 and IFN-γ on γδ T cells after PMA/Ionomycin stimulation. The experiment was repeated 2 times, 3 mice/group.