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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Biotechnol J. 2009 Aug;4(8):1210–1214. doi: 10.1002/biot.200800273

Figure 6.

Figure 6

A calculation of the theoretical maximum concentration of antibodies that could adsorb onto the surface of a microtiter well. The variables can be adjusted for other proteins and uses. This calculation can aid in determining the best dilution of sample and components to use as well as help avoid immeasurable sample oversaturation and conserve components. As expected and shown in figure 5, the actual observed saturation concentration of antibody was below and close to the predicted 2.2 µg/ml maximum.