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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Nat Neurosci. 2009 Dec 27;13(2):180–189. doi: 10.1038/nn.2471

Figure 5. Silencing HDAC1, but not other isoforms prevents neurite beading.

Figure 5

(a) Primary neurons were infected with Hdac1-shRNA lentiviral particles and then exposed to 50μM glutamate/200ng/ml TNF-α for 2 hours and processed for immunocytochemistry with anti-HDAC1 (green) and NFs (red) antibodies. DAPI (blue) was used as nuclear counterstain. Scale bar 10μm for low magnification and 2 μm for high magnification (High mag.). (b) Cultured neurons were infected with class I (Hdac2, Hdac3 and Hdac8) and class II (Hdac4 and Hdac6) Hdac-shRNA lentiviral particles. 72 hours after infection, cultures were exposed to 50μM glutamate/200ng/ml TNF-α for 2 hours and then processed for immunocytochemistry with antibodies specific for HDAC2, HDAC3, HDAC4, HDAC6, or HDAC8 (green) and NFs (red). Scale bar 10 μm for low and 2 μm for high magnification.