Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Nat Neurosci. 2009 Dec 27;13(2):180–189. doi: 10.1038/nn.2471

Figure 7. HDAC1 binding partners in demyelinating region of cuprizone-treated mice.

Figure 7

(a) Validation of the MALDI-TOF results in vivo. Total proteins from demyelinated (Corpus callosum) and (b) non-demyelinated (Spinal cord) regions of cuprizone-treated (Cupri4w) or control mice were immunoprecipitated with anti-HDAC1, HDAC4, or HDAC6 antibody and probed with the indicated antibodies. Shown are representative results of 3 independent experiments, each comparing 3 mice for each condition. Note that HDAC1 forms protein complexes with proteins involved in axonal transport only in demyelinated regions. The expression levels of CNPase, an enzyme critical for myelination process, was used to verify the occurrence of demyelination, while expression levels of HDAC1, HDAC4 and HDAC6 were used to confirm the efficiency of the immunoprecipitation.