Table 1.
Antigen | Immunogen | Manufacturer (catalog no.), Host species | Dilution |
---|---|---|---|
PCP2 | NT peptide of mouse PCP2: magspdqegffnllthvqgdr | Prepared by Rod Feddersen, Rabbit polyclonal | 1:1000 |
Gαo | Purified bovine protein | Millipore (MAB3073; clone 2A), Mouse monoclonal | 1:300 |
Gγ13 | aa 47–59 of mouse Gγ13: cflnpdlmknnpwv | Gift of Robert Margolskee, Mount Sinai School of Medicine, Rabbit polyclonal | 1:500 |
PKCα | Purified bovine PKCα | Sigma-Aldrich (P 5704; Clone MC5), Mouse monoclonal | 1:500 |
CCK precursor | G6-gly peptide: ygwmdfg | Gift of John DelValle, University of Michigan, Rabbit polyclonal | 1:1000 |
CD15 | U-937 histiocytic cell line (human) | ATCC, Manassas, VA (HB-78-MMA)*, Mouse monoclonal | 1:1-2 |
mGluR6 | CT of human protein: c-katstvaappkgedaeahk | Prepared by our lab, Rabbit polyclonal | 1:100 |
Blue opsin | A peptide within the NT of human blue-opsin: efylfknissvgpwdgpqyh | Santa Cruz Biotechnical Inc. Europe (OPN1SW (N-20):sc-14363), Goat polyclonal | 1:50 |
ChAT | Human placental enzyme | Millipore/Chemicon (AB144P-200ul) Goat Polyclonal | 1:200 |
Catalog number is for the hybridoma cell line; antibody was collected from the medium according to manufacturer's protocol.
CT, C-terminus; NT. N-terminus.