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. Author manuscript; available in PMC: 2011 Jan 13.
Published in final edited form as: J Am Chem Soc. 2010 Jan 13;132(1):48–49. doi: 10.1021/ja909325m

Figure 2. The PGT homopolymerization (A) and acceptor site (B) assays.

Figure 2

A) PGTs contain two substrate binding sites; one for a glycosyl donor (D) and one for a glycosyl acceptor (A). During initiation, one LP2 serves as the donor and another as acceptor. Following the first turnover (second row), the elongating strand is the donor and LP2 is the acceptor. In this assay, LP2 must react from both donor and acceptor site to form product. B) For the acceptor site assay, GLP4 (3b) is the donor and the acceptor is varied. Formation of GLP6 demonstrates that the LP2 analog (1c–g) can be an acceptor.