Figure 2.
UV/Vis spectra for the reaction between HOCbl (0.050 mm) and DEA-NONOate (10.0 mm) at pH 10.80. (Spectra taken every 10.0 min, 0.30 m CAPS, I=1.0m (NaCF3SO3), 25.0°C.) Isosbestic points occur at 341, 370, and 498 nm. Inset a) First and last spectra. The final product is NOCbl. b) Fit of the absorbance data at 356 nm (A356) versus time to a first-order rate equation, giving kobs=(1.91±0.01)×10−3 min−1.