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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2003 Oct;44(10):4489–4496. doi: 10.1167/iovs.03-0041

Figure 8.

Figure 8

The weakly ICG-fluorescent cells were confirmed to be neither T lymphocytes nor B lymphocytes. Human whole peripheral blood was incubated with ICG for 1 hour, and the buffy coat fraction containing leukocytes was isolated and stained with mAb against the T lymphocyte marker CD3 or with mAb against the B lymphocyte marker CD19. (A) The weakly ICG fluorescent cells were CD3−, thus not T lymphocytes. The fluorescence intensities of ICG and cy5-conjugated anti-CD3 mAb in each cell were measured and shown in a two-parameter dot plot. (B) The weakly ICG-fluorescent cells were CD19−, thus not B lymphocytes. The fluorescence intensities of ICG and cy5-conjugated anti-CD19 mAb in each cell were measured and shown in a two-parameter dot plot. Similar results were obtained when whole peripheral blood was incubated with ICG and stained with both mAbs.