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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Circ Res. 2006 Feb 23;98(5):e39–e47. doi: 10.1161/01.RES.0000215436.92414.1d

Figure 11.

Figure 11

Intracellular metabolism of adenosine to AMP is essential for phosphorylation of AMPK. Representative Western blots of HUVEC pre-treated for 30 minutes with either an inhibitor of adenosine kinase, AMDA (10 μmol/L) or inhibitor of adenosine deaminase, EHNA (10 μmol/L), and stimulated with adenosine (ADO) (25 μmol/L, 5 minutes). Cell lysates were immunoblotted with anti-P-AMPK (Thr-172), anti-AMPK-α and anti-P-ACC antibodies. Values are means ± S.E.M of 3-4 independent experiments. *P<0.05, compared to control.