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Changes in AMP:ATP ratio do not affect AMPK activation by extracellular nucleotides and adenosine. HUVEC were stimulated with (A) 100 μmol/L ADP or (B) 25 μmol/L adenosine for 5 and 10 minutes and concentrations of intracellular AMP and ATP were measured by HPLC. Data are expressed as means ± S.E.M of 3 independent experiments. P>0.05 compared to control.
