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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Circ Res. 2006 Feb 23;98(5):e39–e47. doi: 10.1161/01.RES.0000215436.92414.1d

Figure 6.

Figure 6

Role of P2X and P2Y11 receptors in AMPK phosphorylation. HUVEC were stimulated with ATP (1 mmol/L, 5 minutes) with and without pre-incubation with KN62 (1 μmol/L, 20 minutes), the antagonist of P2X7 receptor. Cells were also treated with BzATP (100 and 300 μmol/L, 5 minutes), an agonist for P2X and P2Y11 receptors. Cell lysates were analyzed by Western blotting using antibodies to P-AMPK (Thr-172), AMPK-α and P-ACC. Results obtained from 3 independent experiments are presented as means ± S.E.M. **P< 0.01 compared to control.