TABLE 1.
Dependency of SR-A-Mediated Cell Spreading on PI3K
| 10 min
|
120 min
|
|||||
|---|---|---|---|---|---|---|
| Control | Wortmannin | LY294002 | Control | Wortmannin | LY294002 | |
| Cell surface area (μm2) | Cell surface area (μm2) | |||||
| SR-A | 130.1 ± 16.8 | 119.3 ± 4.9 | 130.1 ± 16.8 | 705.6 ± 27.7 | 197.4 ± 16.3a | 158.8 ± 11.4a |
| SR-AΔ1–49 | 358.3 ± 27.3a | 362.7 ± 8.4a | 336.5 ± 26.9a | 751.7 ± 59.6 | 735.6 ± 45.0 | 719.3 ± 19.4 |
| SR-AQNAN | 442.0 ± 52.1a | 434.9 ± 21.6a | 412.6 ± 40.9a | 701.5 ± 45.8 | 773.8 ± 44.1 | 728.5 ± 13.7 |
Cell surface area was assessed using Alexa-Fluor568-phalloidin to stain cells and quantified using image analysis software. Data represent the mean ± sem obtained from images representing at least three independent experiments.
a
P < 0.05 compared with the untreated SR-A control value for that time.