Figure 1.

Immunoblot analysis of tenascin-W and tenascin-C expression in glioma protein extracts. Protein extracts of glioma or healthy brain tissues were separated by SDS-PAGE and immunoblotted for tenascin-W and tenascin-C. Blots were also probed for actin or vinculin to check equal loadings of the samples. A) Healthy brain tissue extracts (N1 and N2) and glioblastoma extracts (lanes 1–29). B) astrocytoma extracts (lanes 1–5). C) Oligodendroglioma extracts (lanes 1–4). Note the absence of tenascin-W in healthy brain tissues. In contrast, the majority of the glioma extracts show expression of tenascin-W. Tenascin-C is also expressed in most of the glioblastomas and astrocytomas, but much less in oligodendrogliomas. Tenascin-C is also detectable in healthy brain tissues.