Figure 3.

Double immunofluorescence staining of tenascin-W or tenascin-C with von Willebrand factor in normal brain and glioblastoma. Cryosections of control brain (A, B) and glioblastomas (C–F) were used for fluorescent detection of tenascin-W (TNW; green; A, C, E), tenascin-C (TNC; green; B, D, F) and von Willebrand factor (vWF; red). Note the normal, typical network of capillaries in normal brain (A, B), compared to the dense network observed in glioblastomas (C, D). There is a complete overlap between TNW and vWF stainings (E), in contrast to the partial overlap between TNC and vWF (F). Scale bars = 50 μm.