Figure 3.
NTD, PKD, GAP1, and GAP2b domains are required for the immunoreactivity of HMB50 or NKI/beteb. EGFP constructs used in this study are shown. EGFP-NP12 contains EGFP and the NTD, PKD, GAP1, and GAP2 domains. EGFP-NP12a contains EGFP and the NTD, PKD, GAP1, and GAP2a domains. EGFP-NP12b contains EGFP and the NTD, PKD, GAP1, and GAP2b domains. EGFP-N196–254P12b contains part of the NTD (aa 196–254), PKD, GAP1, and GAP2b domains. HeLa cells overexpressing EGFP-NP12, EGFP-NP12a, EGFP-NP12b, and EGFP-N196–254P12b were fixed and stained with HMB50 or NKI/beteb and then were reacted with Alexa Fluor 594 or 647, respectively. Scale bars = 20 μm.