Figure 6.

ΔKRG or ΔGAP3 does not abrogate the secretion of Pmel17. A) HeLa cells overexpressing mutants of Pmel17, including ΔKRG, ΔGAP3, ΔTM, and ΔCTD, were radiolabeled and chased for 4 h. Cell lysates and medium were immunoprecipitated with NKI/beteb and then analyzed as described previously. B) HeLa cells overexpressing Pmel17-i and ΔGAP3 were radiolabeled and chased for 4 h. Cell lysates and medium were immunoprecipitated with NKI/beteb, then analyzed as described previously. Note that film was developed after a relatively long exposure time to analyze faster-migrating bands in the medium of Pmel17-i and Pmel17-ΔGAP3. C) HeLa cells overexpressing Pmel17-i and ΔS2 were radiolabeled and chased for 4 h; cell lysates and medium were immunoprecipitated with NKI/beteb, then analyzed as described previously.